Biolegend, 940201, Purified anti-ERG Antibody, 25μg

ERG (ETS-related gene) is a member of the erythroblast transformation-specific (ETS) family of transcription factors. Expressed in the mesoderm and endothelium of embryos, ERG is vital to embryonic cell differentiation as well as regulation of endothelial homeostasis, a role it sustains beyond the embryonic stage. Through transcriptional regulation of VE-cadherin, ICAM-2, and Claudin-5, ERG maintains endothelial junctional integrity and stability and regulation of the endothelial cytoskeleton through its interactions with HDAC6 and RhoJ. ERG is vital to angiogenesis, bone development, platelet adhesion, urogenital tract development, vascular stability, and proper hematopoiesis regulation. It plays a large role in cellular response to vascular inflammation, endothelial cell barrier function, and inhibition of vascular inflammation. These functions are accomplished largely through ERG-mediation of the Wnt/β-catenin signaling pathway by means of transcriptional regulation of FZD4 and VE-cadherin by ERG and subsequent promotion of β-catenin stability. This in turn promotes endothelial cell proliferation. ERG fusion proteins resulting from chromosomal translocations cause ERG to behave as a proto-oncogene, contributing to prostate cancer, megakaryoblastic leukemia, and Ewing’s sarcoma.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human ERG protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa (low expressorl) and MOLT-4 (high expressor) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in robust ERG staining. This clone does not recognize mouse ER. When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor ERG staining. True-Nuclear™ Transcription Factor Buffer Set gave a better signal to noise ratio at all tested concentrations when compared to the True Phos perm buffer.
RRID: AB_2892503 (BioLegend Cat. No. 940201) AB_2892503 (BioLegend Cat. No. 940202)
Structure: ERG is a 449 amino acid protein with an expected molecular weight of 54 kD.
Distribution: Ubiquitously expressed/ Nucleus
Function: Transcription factor
Cell Type: Endothelial cells
Biology Area: Cell Biology, Ubiquitin/Protein Degradation
Antigen References: 1. Adamo P, et al. 2016. J. Biol. Chem. 35:4013 2. Birdsey G, et al. 2015. Dev. Cell. 32:82 3. Shah A, et al. 2016. Vascul. Pharmacol. 86:3
Gene ID: 2078
UniProt: View information about ERG on UniProt.org
Clone: W19256A
Regulatory Status: RUO
Other Names: ETS-Related Gene, Transcriptional Regulator ERG, ETS Transcription Factor ERG, Erythroblast Transformation-Specific Transcription Factor ERG Variant 10, p55
Isotype: Rat IgG2a, κ