Biolegend, 941302, Purified anti-TIF1β (KAP-1, TRIM28) Antibody, 100μg

TIFβ (transcription intermediary factor 1-beta) is an 89 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant mouse TIF1β protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: - BioLegend offers two monoclonal antibodies, clones 20A1 and W19027A that bind TIF1β (KAP-1, TRIM28). 20A1 is a mouse IgG1, κ isotype; W19027A is a rat IgG2a, κ isotype Both clones detect human and mouse TIF1β by western blot; 20A1 binds TIF1β with a higher affinity than W19027A for this application, but also shows a higher background Both clones are validated for immunocytochemistry on human cells W19027A is validated for immunoprecipitation, 20A1 has not been tested for this application - Clone W19027A was tested for immunocytochemistry using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. Both methods were compatible with TIF1β staining.
RRID: AB_2888901 (BioLegend Cat. No. 941301) AB_2888901 (BioLegend Cat. No. 941302)
Structure: TIF1β is an 835 amino acid protein with a predicted molecular weight of 89 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Chromatin remodeling
Antigen References: 1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366. 2. Schultz DC, et al. 2002. Genes Dev. 16:919. 3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859. 4. Friedman JR, et al. 1996. Genes Dev. 10:2067. 5. Messerschmidt DM, et al. 2012. Science 335:1499. 6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.
Gene ID: 10155
UniProt: View information about TIF1beta on UniProt.org
Clone: W19027A
Regulatory Status: RUO
Other Names: Tripartite Motif Containing 28; RING Finger Protein 96; Transcription Intermediary Factor1-β, KRAB [Kruppel-Associated Box Domain]-Associated Protein 1; KRAB-Associated Protein 1
Isotype: Rat IgG2a, κ