CST, 35298S, Arginase-1 (D4E3M™) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying Arginase1. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Arginase-1 (D4E3M^™) Rabbit Monoclonal Antibody (Alexa Fluor^® 555 Conjugate) (CST #35298) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Arginase-1 (D4E3M™) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein. Background L-arginine plays a critical role in regulating the immune system (1-3). In inflammation, cancer, and certain other pathological conditions, myeloid cell differentiation is inhibited leading to a heterogeneous population of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs). MDSCs are recruited to sites of cancer-associated inflammation and express high levels of arginase-1 (4). Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine and urea (5). Thus, MDSCs increase the catabolism of L-arginine resulting in L-arginine depletion in the inflammatory microenvironment of cancer (4,6). The reduced availability of L-arginine suppresses T cell proliferation and function and thus contributes to tumor progression (4,6). Arginase-1 is of great interest to researchers looking for a therapeutic target to inhibit the function of MDSCs in the context of cancer immunotherapy (7). In addition, research studies have demonstrated that arginase-1 distinguishes primary hepatocellular carcinoma (HCC) from metastatic tumors in the liver, indicating its value as a potential biomarker in the diagnosis of HCC (8,9). Alternate Names ARG1; ARGI1; arginase 1; Arginase-1; arginase, liver; Liver-type arginase; Type I arginase Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG