CST, 35446W2, FoxO1 (C29H4) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Red 780 Conjugate)

Monoclonal Antibody for studying FoxO1a. Highly specific and rigorously validated in-house, FoxO1 (C29H4) Rabbit Monoclonal Antibody (SignalFlex^™ mFluor^™ Red 780 Conjugate) (CST #35446) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity FoxO1 (C29H4) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate) detects endogenous levels of total FoxO1 protein. The antibody does not detect exogenously expressed family members FoxO3a or FoxO4. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a GST-fusion protein corresponding to carboxy-terminal residues of human FoxO1. Background The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). Alternate Names FKH1; FKHR; forkhead box O1; forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG