CST, 47414T, Whitlow/218 Linker (F2G3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for WB,IP,IHC,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Whitlow/218 Linker (F2G3S) Rabbit Monoclonal Antibody (CST #47414) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99898 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity Whitlow/218 Linker (F2G3S) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker. This antibody cross-reacts with a protein of unknown identity at approximately 35 kDa in Jurkat cell extracts. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide. Background The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 55 Source/Isotype: Rabbit IgG