Proteintech, 11541-1-AP, Ig Lambda Light Chain Polyclonal antibody

Size: 20ul / 150ul The Ig Lambda Light Chain (11541-1-AP) by Proteintech is a Polyclonal antibody targeting Ig Lambda Light Chain in WB, IHC, IF-P, ELISA applications with reactivity to human samples 11541-1-AP targets Ig Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma tissue, human spleen tissue Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Positive FC (Intra) detected in: Ramos cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:10000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information This antibody detects the lambda light chain of human immunoglobulin. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2064 Product name: Recombinant human IGL- protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC020233 Sequence: MAWSPLLLTLLAHCTGSWAQSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYGNSNRPSGVPDRFSGSKSGTSASLAITGLQAEDEADYYCQSYDSSLSGFVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS Predict reactive species Full Name: immunoglobulin lambda locus Calculated Molecular Weight: 237 aa, 25 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC020233 Gene Symbol: IgG Lambda Light Chain Gene ID (NCBI): 3535 RRID: AB_2877775 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.