Proteintech, 11625-1-AP, CDCA4 Polyclonal antibody

Size: 20ul / 150ul The CDCA4 (11625-1-AP) by Proteintech is a Polyclonal antibody targeting CDCA4 in WB, ELISA applications with reactivity to human, mouse samples 11625-1-AP targets CDCA4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148]. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2214 Product name: Recombinant human CDCA4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC025263 Sequence: MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLNRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET Predict reactive species Full Name: cell division cycle associated 4 Calculated Molecular Weight: 241 aa, 26 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC025263 Gene Symbol: CDCA4 Gene ID (NCBI): 55038 RRID: AB_2260414 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BXL8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.