Proteintech, 12063-1-AP, KRAS Polyclonal antibody

Size: 20ul / 150ul The KRAS (12063-1-AP) by Proteintech is a Polyclonal antibody targeting KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, mouse brain tissue, rat brain tissue Positive IP detected in: HeLa cells, HGC-27 cells, MKN-45 cells, mouse brain tissue Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human kidney tissue, human pancreas cancer tissue, mouse kidney tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, chicken, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM Predict reactive species Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Calculated Molecular Weight: 188 aa, 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC013572 Gene Symbol: KRAS Gene ID (NCBI): 3845 RRID: AB_878040 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01116 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.