Proteintech, 32235-1-AP, NACA Polyclonal antibody

Size: 20ul / 150ul The NACA (32235-1-AP) by Proteintech is a Polyclonal antibody targeting NACA in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 32235-1-AP targets NACA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, U-87 MG cells, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37704 Product name: Recombinant human NACA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC106041 Sequence: MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM Predict reactive species Full Name: nascent polypeptide-associated complex alpha subunit Calculated Molecular Weight: 215 aa, 23 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC106041 Gene Symbol: NACA Gene ID (NCBI): 4666 RRID: AB_3670227 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q13765 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.