Proteintech, 60230-1-Ig, FUT9 Monoclonal antibody

Size: 20ul / 150ul The FUT9 (60230-1-Ig) by Proteintech is a Monoclonal antibody targeting FUT9 in WB, IHC, IF-P, ELISA applications with reactivity to human, pig, mouse, rat samples 60230-1-Ig targets FUT9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse, rat samples. Tested Applications Positive WB detected in: pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human breast cancer tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes. Specification Tested Reactivity: human, pig, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8298 Product name: Recombinant human FUT9 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 32-359 aa of BC036101 Sequence: PTNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWFWN Predict reactive species Full Name: fucosyltransferase 9 (alpha (1,3) fucosyltransferase) Calculated Molecular Weight: 359 aa, 42 kDa Observed Molecular Weight: 44-46 kDa GenBank Accession Number: BC036101 Gene Symbol: FUT9 Gene ID (NCBI): 10690 RRID: AB_11183046 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y231 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.