Product Description
Size: 20ul / 150ul
The RBP5 (68126-1-Ig) by Proteintech is a Monoclonal antibody targeting RBP5 in WB, IF/ICC, ELISA applications with reactivity to Human, pig samples
68126-1-Ig targets RBP5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.
Tested Applications
Positive WB detected in: pig kidney tissue, pig liver tissue, pig liver tisse
Positive IF/ICC detected in: HepG2 cells, A431 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
Background Information
RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).
Specification
Tested Reactivity: Human, pig
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag24562 Product name: Recombinant human RBP5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-135 aa of BC029355 Sequence: MPPNLTGYYRFVSQKNMEDYLQALNISLAVRKIALLLKPDKEIEHQGNHMTVRTLSTFRNYTVQFDVGVEFEEDLRSVDGRKCQTIVTWEEEHLVCVQKGEVPNRGWRHWLEGEMLYLELTARDAVCEQVFRKVR Predict reactive species
Full Name: retinol binding protein 5, cellular
Calculated Molecular Weight: 135 aa, 16 kDa
Observed Molecular Weight: 16-18 kDa
GenBank Accession Number: BC029355
Gene Symbol: RBP5
Gene ID (NCBI): 83758
RRID: AB_2935257
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein G purification
UNIPROT ID: P82980
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
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