Waters, 176001909, ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pk

ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115709
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 3 pk

Additional Information
ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pk With a focus on innovative lab equipment and stationary phases for Hydrophilic Interaction Chromatography (HILIC) to overcome the challenge of retaining and separating extremely polar compounds, ACQUITY UPLC BEH Amide Columns use the novel ethylene bridged hybrid (BEH) to enable a new dimension in stability and versatility. This is done using the chemically stable, trifunctionally bonded amide phase. ACQUITY UPLC BEH Amide Columns facilitate the ability to achieve exceptional retention of polar analytes spanning a wide range in polarity, structural moiety and, pKa through the use of a wide range of mobile phase (2-11). In addition to the enhanced retention of polar analytes and metabolites, these columns provide increased mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), and orthogonal selectivity when compared to reversed-phase materials. The ACQUITY UPLC BEH Amide Column is an excellent tool for the analysis of carbohydrates, such as monosaccharides, disaccharides, oligosaccharides, and polysaccharides. The increased chemical stability of BEH particles enables the use of both high pH and high temperature to collapse reducing sugar anomers, shortening analysis time, and improving MS detection. The BEH particle also provides exceptional column lifetime, improving assay robustness. Lifetime can be extended further through the use of an ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk . Unlike the amine-based columns used for carbohydrate analysis, the ACQUITY UPLC BEH Amide column is not vulnerable to Schiff-based formation, leading to improved quantitation accuracy. The 1.7 µm particle size in these columns enables high resolution, high-speed analysis of carbohydrates in complex sample matrices.

FAQ
What Is The Difference Between Amides And Amines? Amines and amides are both forms of molecules.
When nitrogen is introduced into an organic framework, there are two families of molecules or functional groups that can be formed. Compounds that contain a nitrogen atom bonded in a hydrocarbon framework are classified as amines, while compounds that have a nitrogen atom bonded to one side of a carbonyl group are classified as amides.
Amines are a basic functional group and can combine with carboxylic acids in a condensation reaction to form amides.