Thermo Fisher, EP0753, Maxima H Minus Reverse Transcriptase (200 U/μL)

4 x 10,000 Units Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed throughin vitroevolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT.
The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol.
Features of Maxima H Minus Reverse Transcriptase include:
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotidesApplications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• Analysis of RNA by primer extensionRelated products
•Maxima H Minus Reverse Transcriptase
•Maxima H Minus First Strand cDNA Synthesis Kit
•Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase
•Maxima H Minus cDNA Synthesis Master Mix
•Maxima H Minus cDNA Synthesis Master Mix, with dsDNaseConcentration: 200 U/μL
Final Product Type: First-Strand cDNA
Format: Stand-alone Enzyme
Optimal Reaction Temperature: 50°C to 55°C
Quantity: 4 x 10,000 Units
Reaction Format: Separate components
Reagent Type: Reverse Transcription
Reverse Transcriptase: Maxima H Minus
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Size (Final Product): Up to 20 kb
Starting Material: RNA
Technique: Reverse Transcription
GC-Rich PCR Performance: High
Reaction Speed: 15 to 30 min.
Unit Size: Each