Sartorius Mixing for Bioprocess: From 5 L to 3,000 L

Meet Sartorius Mixing—an integrated, single-use platform built around Flexsafe® Pro Mixer bags, the mobile Pro Mixer drive unit, and Palletank® for Mixing vessels. The levitating magnetic impeller design delivers gentle, low-shear mixing for sensitive biologics while removing clean-in-place steps and cutting changeover time. Systems scale from benchtop (5–20 L) to production (up to 3,000 L), so your mixing strategy stays consistent from development to fill-finish. 

How to Choose Your Sartorius Single-Use Mixing System

Picking the right setup takes three quick decisions:

1. Working volume. Choose a bag size that keeps your typical fill at ~70–80% of nominal volume. Bench work starts at 5–20 L; production runs use 200–3,000 L. 
   
2. Process & fluid. Powder hydration for buffers/media, in-process hold and gentle homogenization, or final formulation? Match ports, sampling, and transfer lines accordingly. 
   
3. Hardware options. Pair the single-use bag with a Pro Mixer drive unit and the right Palletank: single-walled, with weighing, jacketed, or jacketed with weighing (EU PED or US ASME variants). 
   

Tip: If powders are your bottleneck, prioritize top-port access and accessories (powder bag holder, tubing/filter holders) to speed charging and closed transfers.

Product Families: Flexsafe® Pro Mixer Bags, Pro Mixer Drive & Palletank® Vessels

Flexsafe® Pro Mixer Bags

Single-use mixing bags with integrated magnetic impeller assemblies (central at 5–1,000 L; off-center at 1,500–3,000 L) and top ports for contained powder transfer. Designed for fast setup, easy sampling, and predictable scale-up.

Pro Mixer Drive & Controls

Cart-mounted drive that magnetically levitates and spins the impeller—no shaft or bearings contacting product. Local or recipe-based control, 20–750 rpm, and guidance from bag install to end of run.

Palletank® for Mixing (Vessels)

Stainless vessels purpose-built for Pro Mixer bags: single-walled, with weighing, jacketed, or jacketed with weighing—each available across volumes from 50 to 3,000 L in EU (PED) and North American (ASME) versions. 

Benchtop Mixers (5–20 L)

Modular base unit with interchangeable 5, 10, and 20 L holders—ideal for development and pilot work with the same impeller technology used at scale.

Bioprocess Applications: Buffer/Media Preparation, Low-Shear Mixing & Final Formulation

From benchtop trials to production-scale batches, Sartorius single-use mixing keeps fluids homogeneous, accelerates powder hydration, and protects shear-sensitive biologics. Use the guide below to map each unit operation to the right Flexsafe® Pro Mixer bag, Pro Mixer drive, and Palletank® configuration.

• Buffer preparation. Rapid powder hydration and homogenization in a closed system; support for top-port powder charging, minimal foaming, and consistent conductivity targets.
• Media preparation. Scalable from small development runs to large production lots; gentle mixing for supplements and temperature-sensitive components, with straightforward transfer to seed/bioreactor steps.
• In-process, low-shear mixing. Maintain uniformity in hold tanks between clarification, TFF, and chromatography; prevent settling without damaging proteins or other large molecules.
• Final formulation & hold. Inline access for pH, conductivity, and temperature monitoring; aseptic sampling and sterile transfers to filtration and filling.
• pH adjustment & component addition. Dose acids/bases or concentrates via closed manifolds; achieve target pH and osmolality without exposing product.
• Intermediate hold & transfer. Use integrated weighing for gravimetric control; stage intermediates for downstream steps with predictable mixing and drawdown.
• Scale-down/scale-up continuity. Apply the same impeller principles across volumes for comparable mixing profiles during tech transfer.

Key Technologies & Compatibility: Levitating Impeller, Single-Use Sensors, ASME/PED Options

Sartorius Mixing pairs Flexsafe® Pro Mixer bags with a magnetically levitated impeller and purpose-built Palletank® vessels. The result is gentle, efficient, and compliant single-use mixing that drops into upstream or downstream bioprocess trains with minimal validation overhead.

• Levitating magnetic impeller (low-shear). No shaft or bearings in product contact—reduced particle risk, gentle mixing for biologics, and simpler changeovers.
• Inline sensor readiness. Compatible pathways for single-use pH, conductivity, and temperature sensors to support PAT, batch records, and recipe-based control.
• Configurable ports & manifolds. Closed powder charging via top ports, sterile sampling, and aseptic quick-connects for transfers and filtration; sanitary clamp interfaces on the vessel side.
• Bag film & sterility. Gamma-irradiated Flexsafe® film with validated welds and connectors for secure, closed mixing from make-up to hold.
• Drive & controls. Pro Mixer drive provides guided setup, broad rpm range, and local/recipe operation; integrates with weighing for gravimetric make-up and dosing.
• Palletank® variants (regional compliance). Single-walled, with weighing, jacketed, and jacketed-with-weighing options in ASME (North America) and PED (EU) versions to match site standards.
• Utilities & skid integration. Compatible with upstream seed/media prep, TFF/NFF systems, and chromatography skids; mobile cart options ease cleanroom layout changes.
• Predictable scale-up. Consistent geometry and impeller technology from 5–3,000 L supports reproducible process transfer across development and manufacturing.

Featured Models, SKUs & Specifications | Sartorius Mixing

Use the shortlist below to quickly match your process to a standard Sartorius setup. Each entry highlights a representative SKU with working volume, impeller type, port configuration, sensor readiness, and typical use, so you can validate fit before exploring the full catalog. Models span benchtop to 3,000 L Palletank® systems and include EU (PED) and North American (ASME) vessel options. If you need closed powder charging, integrated weighing, or jacketed heat control, check the notes column to narrow choices in seconds.

FAQs

1. What’s the main difference between Sartorius Mixing and a wave-type bioreactor?

Mixing systems are for media/buffer prep, in-process hold, and formulation with magnetic impeller mixing; wave bioreactors are for cell culture. Different goals, different hardware.

2. How do I size a bag vs. my batch volume?

Plan nominal volume so your typical fill sits around 70–80% to leave headspace for powder addition and mixing efficiency.

3. Can I run sensor-based control?

Yes—bags and Palletanks support single-use pH, conductivity, and temperature pathways for inline monitoring and recipe control. 

4. Do Palletanks include weighing or jackets by default?

They’re optioned by series: single-walled, with weighing, jacketed, and jacketed with weighing in both EU PED and US ASME versions. 

5. Is scale-up predictable?

The platform uses the same levitating impeller principles from 5 L to 3,000 L, supporting consistent process transfer. 

Works Well With

• Normal Flow Filtration (NFF) and Tangential Flow Filtration (TFF) for clarification and UF/DF.
• Chromatography skids and columns for downstream steps.
• Single-Use Storage & Shipping (Flexsafe® 2D/3D) for intermediates and drug substance.

Request a Quote & Lead Time

Ready to spec your Sartorius single-use mixing setup? Contact iRight with your target volume, process step (buffer/media prep, in-process hold, final formulation), required ports/sensors, region standard (ASME or PED), and timeline. Our team will map the right Flexsafe® Pro Mixer bag + Pro Mixer drive + Palletank® combination and send pricing, lead time, and any in-stock alternatives. Contact iRight to get a quote today.