BD, 552727, BD Pharmingen™ Purified Mouse Anti-Human CD21

Alternative Name: CR2; Complement receptor type 2; C3DR; EBV-R; Epstein-Barr virus receptor
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human CR2 Recombinant Protein
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394446
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometry: The 1048 antibody is useful for flow cytometric analysis of CD21/CR2 expressed on cell surfaces. Double staining PBMCs with clone 1048 and BD Biosciences Pharmingen's other CD21 specific clone, B-ly4 (Cat. No. 559867) reveal up to 98% identity of the positive population, indicating that these two clones recognize different epitopes of CR2 (data not shown).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.