Abcam, ab100614, Human NRG1 beta 1 ELISA Kit

Size: 1 x 96Tests
Human NRG1 beta 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NRG1 beta 1 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 50 pg/mL,
Range:82.3 - 20000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.
The ab100614 kit recognises the human HRG beta 1 protein (isoform 6) and has not been tested with other isoforms.
Optimization may be required with urine samples

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NRG1 beta 1 protein also known as neuregulin 1 or NRG1 plays an essential mechanical role in cellular communication and growth. It is part of a family of proteins involved in cell signaling and it exists in several isoforms one being NRG1 beta 1. The mass of NRG1 beta 1 is variable due to its different isoforms typically ranging from 44 to 47 kDa. This protein is expressed in various tissues including the heart brain and other neural tissues indicating its importance in both peripheral and central systems.
Biological function summary
NRG1 beta 1 significantly influences the development and function of the nervous system. It interacts with the ErbB family of receptors to form a complex that plays a role in cellular proliferation and differentiation. This interaction is important for synaptic plasticity and the formation of neuromuscular junctions. NRG1 beta 1 also contributes to the maintenance of heart function by regulating growth and repair processes in cardiac muscle cells.
Pathways
Researchers recognize NRG1 beta 1 as an important component in the PI3K/AKT and MAPK signaling pathways. These pathways are important for regulating cell survival growth and differentiation. Through its binding with ErbB receptors NRG1 beta 1 activates these pathways influencing downstream effectors. Research has shown a relationship between NRG1 and other signaling proteins such as neurotrophins indicating its central role in neurodevelopmental processes.
NRG1 beta 1 links to conditions like schizophrenia and heart failure. Altered NRG1 signaling has been associated with schizophrenia where it interacts with ErbB4 receptors showing changes in cognitive function and synaptic connectivity. In cardiac diseases including heart failure dysregulation of NRG1 beta 1 affects cardiac muscle cells' repair interacting with proteins like ErbB2. These relationships highlight the importance of NRG1 beta 1 in both neural and cardiac health contexts.