Abcam, ab100895, Anti-HCV subtype 1b NS5B antibody [10D6]

Size: 100µg
Mouse Monoclonal POLG antibody. Suitable for IP, ELISA, WB and reacts with Hepatitis C virus samples. Cited in 3 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10D6,
Isotype:IgG1,
Carrier free:No,
Reacts with:Hepatitis C virus,
Applications:WB, ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCV subtype 1b NS5B also known as nonstructural protein 5B is an RNA-dependent RNA polymerase (RdRp) important for Hepatitis C virus replication. This enzyme is an important component of the HCV replication complex facilitating viral RNA synthesis. It has a molecular mass of approximately 66 kDa. NS5B is expressed in infected liver cells where it remains membrane-associated due to its C-terminal tail. Antibodies against HCV NS5B such as anti-HCV are often utilized in research for detection and analysis including HCV Western blot applications.
Biological function summary
This enzyme plays a critical role in the synthesis of viral RNA by using viral RNA as a template. NS5B is not part of a classical protein complex but interacts with other viral proteins in the replication complex including NS3 and NS5A to carry out RNA replication and produce progeny virus. Anti-HCV antibodies can aid in studying these interactions. Enzyme inhibitors targeting NS5B are a major focus in HCV therapeutic development efforts.
Pathways
NS5B participates in the life cycle of HCV specifically the replication phase. The replication of HCV involves multiple stages and NS5B's role is integral in the RNA synthesis step. It interacts with the NS5A protein which modulates RNA replication. The interplay between NS5B and NS5A represents a significant point in the HCV replication pathway influencing viral loads and laboratory studies often use anti-HCV and anti-HCV negative controls in mechanistic research.
HCV subtype 1b NS5B is central in chronic Hepatitis C infection and liver diseases such as cirrhosis and hepatocellular carcinoma. Interference with NS5B's RNA polymerase activity is a proven strategy in antiviral therapy making it a target for drugs like polymerase inhibitors. Its connection to NS5A which also supports viral replication corroborates its relevance in HCV pathology and targeted therapies often include combination treatments that address both proteins to improve patient outcomes.