Abcam, ab100932, Anti-Citrulline antibody

Size: 200µL
Rabbit Polyclonal Citrulline antibody. Suitable for ICC, ELISA and reacts with Modified Amino Acid samples. Cited in 15 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Citrulline.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:ICC, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab100932 reacts specifically with intrapeptidic citrulline independently of the amino acid sequence. It does not react with free citrulline, ornithine or arginine. Citrulline is an un-natural amino-acid and its expression is very low. Factors limiting the detection are the amount of endogenous citrulline within a protein and the accessibility of the antibody to these sites.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Citrulline also known as L-citrulline engages in important roles within the nitrogen balance of the body. It is a non-essential amino acid with a molecular weight of about 175.19 g/mol. Citrulline is not incorporated into proteins but is primarily found in liver and intestinal cells. It serves as an intermediate in the urea cycle an important metabolic pathway related to the disposal of excess nitrogen from the body.
Biological function summary
Citrulline plays a significant part in the body's nitrogen cycle and enhances the production of nitric oxide a vital signaling molecule. Citrulline participates in converting ammonia a byproduct from amino acid breakdown into urea in the liver which the body can then excrete. It does not belong to a complex but acts as a precursor to the amino acid arginine enhancing its levels and promoting vasodilation. Citrulline effects also extend to influencing muscle metabolism and cellular energy production.
Pathways
Citrulline participates in the urea cycle and nitric oxide synthesis pathway. It provides a link between ammonia detoxification and the synthesis of arginine. This link enhances nitric oxide availability which is essential for regulating vascular homeostasis and blood pressure. Additionally citrulline relates to the arginine biosynthesis pathway where it aids the conversion to arginine mediated by enzymes such as argininosuccinate synthetase and argininosuccinate lyase. These interactions with enzymes highlight its integration into metabolic pathways central to the organism’s homeostasis.
Citrulline shows involvement in cardiovascular diseases and urea cycle disorders. Citrulline has emerged as a therapeutic marker for cardiovascular diseases by enhancing nitric oxide production and improving endothelial function. Citrulline’s role in urea cycle disorders links it to conditions where the body's ability to detoxify ammonia is compromised. Furthermore citrulline's relationship with arginine impacts both cardiac health and ammonia detoxification integrating it into therapeutic strategies targeting these conditions.