Abcam, ab101013, Anti-PRDM9 antibody

Size: 200µL
Rabbit Polyclonal PRDM9 antibody. Suitable for ICC, ELISA, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PRDM9.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRDM9. The exact immunogen used to generate this antibody is proprietary information.Q9NQV7,
Specificity:ab101013 is predicted to crossreact with Human 56 kDa PRDM7.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRDM9 also known as Meisetz is a zinc finger protein that acts as a histone methyltransferase. This protein influences the organization and function of chromatin specifically by tri-methylating histone H3 at lysine 4 (H3K4me3). It weighs approximately 89 kDa. PRDM9 is mainly expressed in the reproductive organs where it plays a vital role in meiosis particularly during the early stages of gametogenesis. Its expression is important for initiating meiotic recombination hotspots.
Biological function summary
PRDM9 plays a central role in germ cell development and meiosis. It initiates and regulates the recombination of genetic material during the production of gametes. PRDM9 operates as part of a complex with significant influence on the localization of DNA double-strand breaks thereby ensuring genetic diversity. It changes the chromatin structure around specific genomic regions to create an accessible environment for recombination machinery.
Pathways
The protein plays an influential role in genetic recombination and chromatin modification pathways. In meiotic recombination PRDM9 determines the locations of recombination hotspots by binding to DNA and modifying adjacent histones. It functions alongside proteins such as SPO11 a topoisomerase-like protein which is essential for the initiation of homologous recombination through DNA double-strand breaks.
Mutations or aberrations in PRDM9 can lead to fertility issues and meiotic arrest. Variants of PRDM9 have been associated with azoospermia a condition characterized by a lack of sperm cells. Its interaction with other proteins like SPO11 links PRDM9 to developmental disorders related to faulty meiotic recombination processes highlighting its importance in reproductive health.