Abcam, ab1017, Anti-Aflatoxin B1 antibody [AFA-1]

Size: 250µg
Mouse Monoclonal Aflatoxin B1 antibody. Carrier free. Suitable for ELISA and reacts with Chemical samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Aflatoxin B1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AFA-1,
Isotype:IgG2a,
Carrier free:Yes,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
This product was changed from ascites to tissue culture supernatant on 5th December 2019. Lot numbers higher than GR285242 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aflatoxin B1 (AFB1) is a potent mycotoxin produced by Aspergillus species particularly Aspergillus flavus and Aspergillus parasiticus. It is a small toxic compound with a molecular mass of about 312 grams per mole. Aflatoxin B1 commonly appears in agricultural environments as these fungi contaminate crops like nuts cereals and maize. In biological matrices the molecule often requires detection via sophisticated methods such as aflatoxin ELISA kits which facilitate sensitive and specific measurement of AFB1 levels.
Biological function summary
Aflatoxin B1 exerts its toxic effects by interacting with DNA and inducing mutations that can lead to cancer. It interacts with the cytochrome P450 enzyme complex in the liver where it undergoes bioactivation to form an epoxide. This reactive metabolite can covalently bind to DNA resulting in genotoxic stress. It does not form part of any large protein complexes on its own but relies on enzymatic conversion to exert its biological activity.
Pathways
AFB1 primarily involves the cytochrome P450 enzymatic system. This pathway activates AFB1 into a reactive epoxide linking it to the metabolic pathways of xenobiotic degradation. It is closely related to other cytochrome P450 enzymes such as CYP1A2 and CYP3A4 which also participate in metabolic activation and detoxification of various xenobiotics and drugs influencing their biological impact.
Aflatoxin B1 is strongly associated with hepatocellular carcinoma a primary form of liver cancer due to its genotoxic properties. It is also linked to acute aflatoxicosis symptoms of which include liver damage and impaired immunity. The protein p53 is commonly associated with AFB1-related liver cancer as mutations in p53 are facilitated by aflatoxin-induced DNA damage undermining the cell's repair mechanisms and contributing to oncogenesis.