Abcam, ab101721, Anti-PAX4 antibody

Size: 200µL
Goat Polyclonal PAX4 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Paired box protein Pax-4 aa 150-250.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Paired box protein Pax-4 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.O43316

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab101721 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PAX4 also known as paired box 4 is a protein encoded by the PAX4 gene. This transcription factor has a molecular mass of around 36 kDa. It plays a significant role in the regulation of pancreatic islet development and insulin gene expression. PAX4 is expressed mainly in pancreatic beta cells. Its expression is important for the proper differentiation of these cells from precursor cells and is pivotal during embryogenesis in the formation of pancreatic endocrine cells.
Biological function summary
PAX4 influences cellular activities critical for the development of insulin-producing cells in the pancreas. As a transcription factor it does not work in isolation but interacts with other proteins taking part in complexes that drive gene expression changes. It represses the expression of genes like glucagon ensuring the adequate development and function of insulin-secreting cells. Therefore PAX4 ensures a delicate balance in glucose homeostasis.
Pathways
PAX4 plays a significant part in the insulin signaling pathway and is essential in pathways affecting glucose metabolism. It interacts with RXR and PDX1 which are key proteins in the regulation of insulin and glucose metabolism genes. By controlling these pathways PAX4 contributes to maintaining normal glucose levels and effective insulin function which is critical for energy balance in the body.
Mutations or dysregulation of PAX4 have been linked to type 2 diabetes and maturity-onset diabetes of the young (MODY). These conditions result from the disrupted ability of pancreatic beta cells to produce insulin. PAX4 mutations can affect its interaction with proteins like PDX1 further influencing diabetes pathogenicity. Understanding PAX4 in these contexts can be vital for developing therapeutic strategies that target these pathways and their associated disorders.