Abcam, ab101876, Anti-GPT2 antibody

Size: 200µL
Rabbit Polyclonal GPT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GPT2 protein also known as Glutamyl-Prolyl-tRNA Synthetase 2 is an enzyme essential for the translation process in protein biosynthesis. It functions by charging tRNA molecules with their respective amino acids specifically glutamic acid and proline. The GPT2 protein has a mass of approximately 170 kDa and is expressed in various tissues including muscles and kidneys. This widespread expression suggests its significant role in maintaining protein synthesis in different cellular environments.
Biological function summary
GPT2 enzyme plays an important role in catalyzing the attachment of amino acids to their corresponding tRNA molecules a vital step in ribosomal protein synthesis. As part of the aminoacyl-tRNA synthetases GPT2 forms a complex with other synthetases to ensure accurate decoding of mRNA during translation. These complexes are essential for preserving translational fidelity across diverse biological systems impacting numerous cellular functions.
Pathways
The GPT2 enzyme integrates into the translation pathway where it ensures efficient and precise protein synthesis. Its activity connects closely with the mTOR signaling pathway which regulates cell growth and metabolism in response to nutritional status. GPT2 interacts with other tRNA synthetases and translation initiation factors forming a critical component of the protein synthesis mechanism. This highlights its coordination with other proteins to uphold proper cellular function.
GPT2 has associations with certain mitochondrial diseases due to defects in protein synthesis and metabolism. Errors or mutations in tRNA synthetases including GPT2 can lead to mitochondrial dysfunction and related disorders such as Charcot-Marie-Tooth disease. This connection implicates GPT2 and other proteins in its group like glycyl-tRNA synthetase in these neuropathological conditions. Understanding these associations can facilitate the development of targeted therapies for related diseases.