Abcam, ab10288, Anti-Podoplanin antibody [18H5] - BSA and Azide free

Size: 100µg
Anti-Podoplanin antibody [18H5] - BSA and Azide free (ab10288) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Podoplanin in Western Blot, Flow Cytometry, ICC/IF . Suitable for Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 40 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:18H5,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-Podoplanin antibody [18H5] - BSA and Azide free (ab10288) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Podoplanin?
Anti-Podoplanin [18H5] - BSA and Azide free (ab10288) specifically detects a band for Podoplanin (UniProt: Q86YL7) at a molecular weight of 24kDa.
Trusted by the scientific community
Anti-Podoplanin [18H5] - BSA and Azide free (ab10288) was first used in a scientific publication in 2004 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Podoplanin [18H5] - BSA and Azide free (ab10288) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Podoplanin also known as gp36 and PDPN is a small transmembrane protein weighing approximately 36 kDa. It mainly expresses in lymphatic endothelial cells podocytes and various other tissues like the lung and kidney. It functions as a marker in mesothelial cells and is noticeable in histochemical studies such as mesothelioma using podoplanin staining in immunohistochemistry (IHC). Podoplanin emerges as an important tool in identifying lymphatic vessels and has significant roles in the development of certain tissues.
Biological function summary
The protein participates in maintaining the integrity of cell structures and lymphangiogenesis. This protein does not form part of a larger complex but actively interacts with different molecules. In lymphoid tissues podoplanin contributes to the proper formation of lymphatic channels and assists in platelet aggregation. These actions are pivotal in wound healing and protecting the epithelial cell layers in multiple organs.
Pathways
Podoplanin impacts the platelet activation pathway and the Lymphatic Vessel Development pathway. Its interaction with CLEC-2 (C-type lectin-like receptor 2) triggers downstream signaling leading to changes in platelet morphology and actin cytoskeleton reorganization. Podoplanin’s activation of these pathways links it to key processes that include cell migration and tissue homeostasis TGF-beta interaction also represents a significant relationship within these pathways.
Podoplanin has correlations with conditions like cancer specifically mesothelioma and squamous cell carcinoma. Dysregulation of podoplanin and its pathways may contribute to tumor progression and metastasis. In mesothelioma podoplanin interacts with other proteins like E-cadherin influencing cancer cell migration and adhesion. Its role in disease states highlights the importance of podoplanin IHC as a diagnostic tool aiding in the better understanding and identification of disease pathology.