Abcam, ab10303, Anti-Transportin 1/MIP antibody [D45]

Size: 100µL
Mouse Monoclonal Transportin 1/MIP antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNPO1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:D45,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TNPO1. The exact immunogen used to generate this antibody is proprietary information.Q92973

Product details:
Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Protein A purified from tissue culture supernatant., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Transportin 1 also known as MIP-1 TRN1 or TNPO1 is a protein involved in nuclear transport mechanisms. It functions as a part of the importin-beta family facilitating the transport of cargo proteins into the nucleus by recognizing nuclear localization signals (NLS). Transportin 1 has a molecular weight of approximately 98 kDa and is expressed widely in various tissues. This protein is essential for the nucleocytoplasmic transport operating predominantly in the cytoplasm where it interacts with its cargoes.
Biological function summary
Transportin 1 is an important component in cellular operation by being part of the nuclear pore complex. It mediates the translocation of proteins across the nuclear envelope. The protein plays a role in not just import but also export of numerous cellular regulators and factors influencing gene expression and cell cycle progression. Being part of such complexes places it at the core of many fundamental cellular processes.
Pathways
The actions of Transportin 1 are integral to nuclear-cytoplasmic transport pathways and the Ran-GTPase pathway. It interacts with the small GTPase Ran which provides the necessary energy for active transport and directionality across the nuclear pore complexes. Additionally Transportin 1 shows functional similarity with importin-alpha which expands its role to include overlap in pathway regulation and cargo specificity further embedding it in complex cellular signaling networks.
Transportin 1 has been linked to neurodegenerative conditions and certain cancers. Its role in improper protein transport potentially contributes to the pathogenesis of neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) where mislocalized proteins accumulate improperly. Transportin 1 also associates with the protein TDP-43 known to play a role in ALS and other similar disorders. In cancer abnormal expression of Transportin 1 can alter nuclear transport dynamics affecting tumor suppressor and oncogene distribution thereby contributing to tumorigenesis and cancer progression.