Abcam, ab103427, Anti-MGAT3 antibody

Size: 100µg
Rabbit Polyclonal MGAT3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MGAT3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human MGAT3.Q09327

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MGAT3 also known as GlcNAc-TIII is an enzyme involved in the modification of N-glycans. It catalyzes the addition of N-acetylglucosamine in a beta-14 linkage to core mannose residues on asparagine-linked carbohydrate chains. MGAT3 is approximately 60 kDa in mass and finds expression across various tissues but shows higher levels in the liver pancreas and certain immune cells. By adding a bisecting GlcNAc MGAT3 influences the branching pattern of N-glycans which impacts protein stability and cell signaling.
Biological function summary
The bisecting GlcNAc addition by MGAT3 plays an important role in cellular communication and immune responses. It often works as part of a larger enzymatic system that modifies proteins and lipids on the cell surface. The activity of MGAT3 affects cell-cell interactions and receptor activity. This modification can hinder the action of galectins a family of proteins that bind specific carbohydrate structures affecting processes like cell-cell adhesion and immune cell function.
Pathways
MGAT3 integrates into the glycosylation pathway modifying glycoproteins that play roles in both intracellular and intercellular processes. It forms connections with the TGF-beta signaling pathway by altering receptor glycosylation influencing signal strength and cell fate decisions. MGAT3-related modifications can affect how ligands bind to receptors like EGFR affecting the downstream signaling cascade and cellular outcomes.
MGAT3 modifications have implications for cancer progression and immune system disorders. Altered N-glycan branching patterns can lead to abnormal cell proliferation and immune evasion common in cancers such as liver cancer and pancreatic cancer. MGAT3 links to the activity of other glycosyltransferases and its deregulation may impact proteins like PD-L1 which plays a role in immune checkpoint pathways potentially leading to immune escape and tumor progression.