Abcam, ab104026, Recombinant human Haspin protein

Size: 5µg / 10µg
Recombinant human Haspin protein is a Human Fragment protein, in the 470 to 798 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for WB, FuncS.
Key facts
Purity:>75% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The Specific activity of ab104026 was determined to be 18 nmol/min/mg.,
Accession:Q8TF76,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Haspin also known as Germ Cell-Specific Gene 2 (GSG2) is a serine/threonine-protein kinase with a molecular weight of approximately 86 kDa. Haspin expresses predominantly in the testes but researchers have detected it in other tissues too. This kinase is well recognized for its role during cell division especially in the regulation of chromosome alignment and segregation by phosphorylating histone H3 at threonine 3.
Biological function summary
Haspin kinase plays a significant role in mitosis and meiosis. It orchestrates the proper attachment of microtubules to kinetochores by modifying chromosomal components. Haspin is part of a larger protein complex during cell division working closely with the chromosomal passenger complex to ensure accurate segregation of chromosomes. Its activity ensures the integrity and stability of cellular division processes.
Pathways
Haspin kinase functions centrally in cell cycle regulation and mitotic spindle assembly pathways. It interacts with key proteins in these pathways such as Aurora B kinase. The phosphorylation of histone H3 by haspin establishes a critical docking site for proteins in the chromosomal passenger complex facilitating proper chromosome alignment and spindle checkpoint activation.
Incorrect regulation of haspin activity associates with various cancers and chromosomal instability disorders. Overexpression or mutations in haspin can contribute to tumorigenesis by disrupting normal cell division. Additionally haspin's interaction with the chromosomal passenger complex proteins like survivin influences the development of colorectal cancer.