Abcam, ab104854, Anti-Plzf antibody [5B3]

Size: 100µL
Mouse Monoclonal Plzf antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB16.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5B3,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ZBTB16. The exact immunogen used to generate this antibody is proprietary information.Q05516

Product details:
This product was changed from ascites to supernatant. Lot no's high than GR161367-22 are from Tissue Culture Supernatant

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Plzf also known as promyelocytic leukemia zinc finger protein is a transcriptional repressor critical for its mechanical role in gene regulation. It has a mass of approximately 75 kDa. PLZF shows presence in various tissues notably in hematopoietic stem cells testis and certain neural tissues. It functions by binding to specific DNA sequences influencing transcriptional activity. This protein is part of the large BTB/POZ zinc finger family and it plays an engaged role in chromatin remodeling.
Biological function summary
Plzf helps regulate cell differentiation proliferation and self-renewal. Plzf works within several complexes to control gene expression in hematopoietic and germline stem cells. Through these activities it maintains stem cell quiescence and properly times cell differentiation. Plzf impacts neural crest cell fate during embryogenesis and participates in spermatogonia renewal. This involvement in various cellular processes makes it an important protein for development and stem cell biology.
Pathways
Plzf plays a role in pathways such as the Notch signaling and retinoic acid receptor pathways. Within these pathways Plzf interacts with proteins like RAR (retinoic acid receptor) and Hes-1. These interactions affect transcriptional responses that balance stem cell maintenance and differentiation. Plzf's presence in these pathways underlines its importance in controlling genetic programs associated with development and cellular growth.
Plzf associates closely with acute promyelocytic leukemia (APL) and spermatogenic failure. In APL a specific translocation involving the PLZF gene and the retinoic acid receptor alpha (RARA) gene leads to an oncogenic fusion protein disrupting normal myeloid differentiation. This disruption contributes directly to leukemia pathogenesis. In spermatogenic failure abnormal expression or mutation of Plzf impacts testicular function leading to infertility. Studies examine these connections to understand potential therapeutic targets and treatment strategies.