Abcam, ab106004, Anti-LINE-1 ORF2p antibody

Size: 100µg
Chicken Polyclonal LINE-1 ORF2p antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LINE-1 retrotransposable element ORF2 protein conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Carrier free:No,
Reacts with:Human,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human LINE-1 retrotransposable element ORF2 protein conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.O00370,
Specificity:L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.

Product details:
Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening product.
ab106004 is intended for use in studying control of L1 retrotransposons.

Properties and Storage Information:
Form-Liquid, Purity-IgY fraction, Purification notes-L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LINE-1 ORF2p also known as L1 ORF2 protein is a critical component in the process of retrotransposition. This protein approximately 150 kDa in mass is encoded by the Long Interspersed Nuclear Element-1 (LINE-1) a type of non-long terminal repeat (non-LTR) retrotransposon. LINE-1 ORF2p contains reverse transcriptase and endonuclease activities which are necessary for inserting copies of LINE-1 DNA back into the genome. This protein is mainly expressed in germ cells and early embryonic tissues although its presence is noted in various somatic tissues in certain contexts.
Biological function summary
LINE-1 ORF2p facilitates the mobilization and insertion of LINE-1 elements into new genomic locations an activity integral to maintaining genome plasticity. It is not generally classified as part of a larger protein complex but functions closely with another protein LINE-1 ORF1p. ORF2p's reverse transcriptase domain synthesizes DNA from RNA allowing the LINE-1 element to integrate into the host genome. This action implicates it in genomic rearrangements that can have profound effects on gene expression and genome stability.
Pathways
LINE-1 ORF2p plays a substantial role in genomic and epigenomic pathways particularly those involving DNA synthesis and repair. It interacts with cellular proteins such as DNA repair machinery that modulate its insertion capacity and activity. This protein influences the development and function of cellular pathways through its impact on genomic architecture affecting neighboring gene regulation. The interaction with proteins like the Apurinic/apyrimidinic endonuclease (APE1) is significant as they can regulate LINE-1 insertion events.
LINE-1 ORF2p is associated with cancer and neurodegenerative diseases due to its role in genomic instability. Uncontrolled activity of LINE-1 can lead to mutagenic insertions in oncogenes or tumor suppressor genes contributing to tumorigenesis. Additionally in neurodegenerative diseases inappropriate activation of LINE-1 ORF2p may induce neuronal damage through genomic disarray. Related proteins include PIWI-interacting RNAs (piRNAs) which usually silence LINE-1 activity to prevent genomic chaos.