Abcam, ab106843, Anti-Glutathione Reductase antibody [AP-MAB0866]

Size: 500µg
Rat Monoclonal Glutathione Reductase antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr, Depletion and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Glutathione reductase, mitochondrial protein.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:AP-MAB0866,
Isotype:IgG2,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow Cyt, IHC-P, Depletion, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing Glutathione reductase, mitochondrial protein. The exact immunogen used to generate this antibody is proprietary information.P47791

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-The IgG fraction of culture supernatant was purified by Protein A affinity chromatography and 0.2 μm filtered. Extremely low level of LPS (< 0.002EU/μg IgG), Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione reductase also known as GSR is an enzyme that plays a mechanical role in maintaining the redox balance within cells. This enzyme reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH which is essential for detoxifying reactive oxygen species. Glutathione reductase has a molecular weight of approximately 55 kDa. It is widely expressed in various tissues including the liver where it participates in detoxification processes. The enzyme operates in the cytosol and mitochondria reflecting its necessity across different cellular compartments.
Biological function summary
The enzyme sustains a supply of reduced glutathione which is important for antioxidant defense and cellular protection against oxidative stress. As a critical player in the glutathione system it is not part of a larger complex but interacts significantly within the cellular antioxidant framework. It supports important functions like protein folding and DNA synthesis by keeping the cellular redox environment stable. The enzyme's activity ensures that glutathione an important antioxidant with a molecular weight of 307.32 Da remains in its active form to fulfill its protective roles.
Pathways
The enzyme is deeply integrated into the pentose phosphate pathway which generates NADPH necessary for the reduction of glutathione disulfide. It also connects to the glutathione metabolism pathway where it maintains homeostasis through GSH regeneration. Glutathione reductase intersects with other proteins like glutathione peroxidase (GPx) which uses GSH to reduce hydrogen peroxide displaying a coordinated effort in mitigating oxidative damage.
Deficiency or dysfunction of this enzyme links to conditions such as hemolytic anemia where reduced enzyme activity leads to higher susceptibility to oxidative damage in red blood cells. Additionally its dysfunction associates with neurodegenerative disorders where oxidative stress plays a critical role. The enzyme's function relates to proteins like superoxide dismutase (SOD) in disease pathways where both contribute to managing oxidative stress. Understanding alterations in glutathione reductase activity may offer insights into therapeutic strategies for these oxidative stress-related conditions.