Abcam, ab10689, Anti-hnRNP F+H antibody [1G11]

Size: 100µL
Mouse Monoclonal hnRNP F antibody. Suitable for IHC-P and reacts with Human samples. Cited in 19 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1G11,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Human hnRNP F/H.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from supernatant., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heterogeneous nuclear ribonucleoproteins F and H (hnRNP F+H) are RNA-binding proteins with functions in pre-mRNA processing. Alternate names for these proteins include HNRNPF and HNRNPH. These proteins have molecular masses of approximately 46-49 kDa for hnRNP F and 49-50 kDa for hnRNP H. hnRNP F+H appears widely expressed in various tissues with elevated levels in cells with high transcriptional activity. They possess RNA recognition motifs that directly interact with specific RNA sequences facilitating RNA splicing and stability.
Biological function summary
HnRNP F+H influences mRNA splicing by binding to its substrates and regulating splicing events. The proteins often participate in complexes with other RNA-binding proteins contributing to spliceosome function. They regulate alternative splicing events and affect gene expression important in diverse cellular processes. These activities enable hnRNP F+H to modulate the expression of key genes involved in cell growth differentiation and stress responses.
Pathways
HnRNP F+H integrates into the RNA processing pathway including alternative splicing and mRNA stability modulation pathways. They interact with components of the spliceosome and regulate the splicing of pre-mRNAs affecting the generation of mature mRNAs. hnRNP F+H also closely associates with proteins like serine/arginine-rich splicing factor 1 (SRSF1) and other splicing regulators to influence the splicing landscape. These interactions allow hnRNP F+H to maintain cellular homeostasis and normal cellular function.
HnRNP F+H links to several genetic conditions including spinal muscular atrophy (SMA) and cancer. Abnormal expression or mutations in these proteins can disrupt normal splicing patterns leading to the pathogenesis of these disorders. In SMA altered splicing of the SMN1 gene relates to changes in hnRNP F+H regulation. In cancer aberrant splicing events mediated by hnRNP F+H often involving oncogenes and tumor suppressors can contribute to tumor progression and metastasis. Understanding the role of hnRNP F+H in these diseases provides insight into potential therapeutic approaches.