Abcam, ab108609, Anti-PINCH 1 + PINCH 2 antibody [EP1944]

Size: 100µL / 1mL
Rabbit Monoclonal PINCH 1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1944,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This product has switched from a hybridoma to recombinant production method on 7th September 2023.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PINCH 1 and PINCH 2 also referred to as LIMS1 and LIMS2 are proteins involved in cellular signaling and structural functions. These proteins each have a mass of approximately 37 kDa. They are expressed ubiquitously in various tissues including the heart kidney and smooth muscle among others. Mechanically both PINCH proteins interact with integrin-linked kinase (ILK) and parvin to form the ILK-PINCH-parvin (IPP) complex which plays a significant role in integrin signaling pathways.
Biological function summary
The proteins participate in maintaining cellular adhesion and motility. As members of the IPP complex PINCH 1 and 2 are critical for connecting the extracellular matrix with the actin cytoskeleton. They help transmit signals from the extracellular environment to the cell’s interior influencing cell shape and movement. These proteins are particularly important in the processes of cell survival and tissue development.
Pathways
PINCH proteins engage in integrin-mediated signaling pathways fundamental for cellular responses to mechanical and chemical stimuli. The IPP complex has significant roles in the integrin- and growth factor receptor-mediated pathways especially impacting cell migration and survival. Both proteins link with ILK and through these pathways they interact with various small GTPases that regulate cytoskeletal dynamics.
PINCH 1 and 2 show connections with cancer progression and organ fibrosis. Their expression levels often change in some cancers impacting cell migration and invasion. They also link to fibrosis by influencing extracellular matrix remodeling. Through diseases like cancer PINCH proteins interact with other molecules such as focal adhesion kinase which contributes to altered cell signaling and structure observed in tumor cells and fibrotic tissues.