Abcam, ab109209, Serum Antibody Purification Kit (Protein A)

Size: 1Test / 3Test
Serum Antibody Purification Kit (Protein A) (ab109209) is designed to purify IgG fractions from serum. The kit uses Protein A which has a high affinity for Fc regions of IgG molecules from a variety of species. - All reagents (including Protein A resin) are ready-to-use as supplied. - Up to 20 mg of antibody can be purified in each run. - Compatible with our Lightning-Link ® Antibody Conjugation Kits and Oligonucleotide Conjugation Kit range.
Product details:
Serum Antibody Purification Kit (Protein A) (ab109209)
is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.
The antibody is captured on the resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.
The components of
ab109209
are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits (Gold, Latex, Europium and Magnetic). To purify antibodies for use with our nanoparticle conjugation kits, please use our Antibody Purification Kit - Nanoparticles (
ab204909
ab109209 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.
This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Serum Antibody Purification System. 863-0500 is the same as the 3 tests size. 863-0030 is the same as the 1 test size.
ab109209 contains 1-3 columns, and each can purify up to
of antibody per run.
The volume of sample required will depend on the host species (See Table below):
Species
Normal range IgG(mg/ml)
Suitable Volumes for Product(ml)
Rabbit
12 - 15
1.3 - 1.7
7 - 23
0.9 - 2.9
4 - 10
Sheep/Goat
18 - 24
0.8 - 1.1
2.9 - 4
Ascites Fluid
0.5 - 5
4 - 40

Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C