Abcam, ab109438, Anti-TRPM7 + TRPM6 antibody [EPR4582]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal TRPM7 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4582,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 has been shown to cross react with TRPM6 in WB.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRPM7 and TRPM6 are members of the transient receptor potential melastatin subfamily often referred to as TRP-PLIK or CHAK1 for TRPM7. TRPM7 has a molecular mass of approximately 165 kDa while TRPM6 is roughly 230 kDa. These proteins function as ion channels with kinase activity. They regulate the homeostasis of divalent cations like magnesium and calcium by permitting their entry into cells. TRPM7 is expressed in various tissues including the heart liver and brain while TRPM6 is mostly found in the kidney and intestine.
Biological function summary
TRPM7 and TRPM6 play essential roles in cellular magnesium absorption and homeostasis. They form homo- or heteromeric channel complexes with the combination of TRPM6 and TRPM7 enhancing channel function. These proteins influence critical cellular processes such as proliferation apoptosis and migration by modulating cellular magnesium levels. TRPM7's distinct kinase domain also impacts intracellular signaling pathways through phosphorylation of specific substrates regulating response to cellular stress and differentiation.
Pathways
TRPM7 and TRPM6 modulate cell signaling pathways linked to magnesium-sensitive enzymes and proteins like those involved in the PI3K/Akt signaling pathway. This pathway is integral for cell growth survival and metabolism. TRPM7 interacts with various signaling proteins including PLC and MAPK influencing cellular responses to external stimuli. TRPM6 often partners with TRPM7 to fine-tune these cellular pathways maintaining appropriate ionic balances necessary for cellular functions.
TRPM7 and TRPM6 are linked to magnesium homeostasis-related conditions such as hypomagnesemia and hypertension. Variants in TRPM6 associate with hypomagnesemia with secondary hypocalcemia a genetic disorder affecting magnesium uptake in the intestine. TRPM7 is connected to neurodegenerative diseases given its role in neuronal survival and response to oxidative stress. The interaction of TRPM7 with proteins involved in neurodegenerative pathways like amyloid precursor protein suggests potential involvement in Alzheimer’s disease pathogenesis.