Abcam, ab109446, Anti-HDAC9 antibody [EPR5223]

Size: 20µL / 100µL / 1mL
Anti-HDAC9 antibody [EPR5223] (ab109446) is a rabbit monoclonal antibody detecting HDAC9 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5223,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt (Intra), WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-HDAC9 antibody [EPR5223] (ab109446) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of HDAC9?
Anti-HDAC9 [EPR5223] (ab109446) specifically detects a band for HDAC9 (UniProt: Q9UKV0) at a molecular weight of 111kDa.
Trusted by the scientific community
Anti-HDAC9 [EPR5223] (ab109446) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-HDAC9 antibody [EPR5223] (ab109446) has been confirmed by Western blot testing in HDAC9 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR5223] also available for your convenience: ab109446, Alexa Fluor® 488 -
ab206713
, Alexa Fluor® 647 -
ab207050
, Carrier free -
ab239979
, PE -
ab305791
, APC -
ab305792
, HRP -
ab305793
, Alkaline Phosphatase -
ab308767
, Alexa Fluor® 594 -
ab310482
, Alexa Fluor® 555 -
ab312011
, Alexa Fluor® 568 -
ab312488
, Alexa Fluor® 750 -
ab320953
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC9 also known as Histone Deacetylase 9 is a protein involved in removing acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC9 has a molecular weight of about 127 kDa. It expresses predominantly in the brain muscle tissue and immune cells. This protein belongs to the class IIa family of histone deacetylases and has close structural similarities to other class IIa HDACs like HDAC4 HDAC5 and HDAC7.
Biological function summary
HDAC9 plays a significant role in regulating gene expression by altering the acetylation status of histones. It participates in the formation of large multiprotein complexes that target specific gene promoters. HDAC9's activity impacts various cellular processes including cell cycle progression and differentiation. It is a part of the CoREST complex which interacts with REST corepressor 1 to modulate neuronal gene expression and synaptic plasticity.
Pathways
HDAC9 is actively involved in key cellular signaling cascades. It contributes to the Notch signaling pathway which has a profound impact on cell differentiation and fate decisions. It also plays a role in the NF-kB pathway which is critical for immune response and inflammation. Through these pathways HDAC9 interacts with proteins such as RBP-Jk in the Notch pathway and IKK complex members in the NF-kB pathway indicating its broad influence on cellular mechanisms.
HDAC9 associates with several pathological conditions. It has been linked to cardiovascular diseases such as cardiac hypertrophy and heart failure due to its influence on cardiac muscle gene expression. HDAC9 also relates to various cancers including colon cancer where its dysregulation affects tumorigenesis and cell proliferation. In these contexts HDAC9 interacts with proteins like MEF2 and p300 which are pivotal in cardiac growth and cancer progression respectively.