Abcam, ab109515, Anti-DDX50 antibody [EPR5273]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal DDX50 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5273,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX50 also known as DEAD box polypeptide 50 is an RNA helicase with ATPase activity that unwinds RNA functioning in ribosome biogenesis. It belongs to the DEAD-box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (D-E-A-D). The molecular weight of DDX50 is approximately 82 kDa. This protein expresses mainly in nucleoli within cells where it likely participates in pre-rRNA processing and ribosomal subunit assembly.
Biological function summary
The protein DDX50 plays roles in RNA processing and modification during ribosome production. It does not function alone but as part of a ribonucleoprotein complex that facilitates the maturation of ribosomal RNA. By interacting with other components it assists in the structural rearrangements and modifications necessary for proper ribosome assembly impacting overall cellular protein synthesis.
Pathways
Cellular processes involving ribosome assembly and function depend on proteins like DDX50. It integrates in the ribosome biogenesis pathway influencing the synthesis and assembly of ribosomal subunits. In this pathway DDX50 works with proteins such as DDX21 and NOP56 sharing functional domains to facilitate efficient rRNA modification and processing which are critical to ribosome construction and cellular growth.
Alterations in DDX50 expression or function connect to cancer and some hematological conditions. DDX50's role in ribosome biogenesis makes it a factor in tumorigenesis particularly in cancers where cell proliferation relies heavily on increased protein synthesis. Additionally DDX50 interaction with proteins like NOP56 points to a potential link to dyskeratosis congenita a disorder affecting bone marrow and the skin. Understanding these connections may open doors for developing diagnostic or therapeutic strategies targeting DDX50 and similar proteins.