Abcam, ab11003, Anti-Actin antibody [AC-40] - Loading Control

Size: 50µL
Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for Dot, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Guinea pig, Pig, Hamster, Snail, Carp, Xenopus laevis, Chicken, Human, Dog, Cow, Goat, Sheep, Rabbit, Mouse, Rat samples. Cited in 60 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1 aa 350 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AC-40,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human, Sheep, Pig, Xenopus laevis, Rat, Snail, Rabbit, Dog, Mouse, Chicken, Hamster, Guinea pig, Cow, Carp, Goat,
Applications:Dot, WB, IHC-P, ICC/IF, ELISA, IHC-FoFr, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACTA1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P68133,
Epitope:Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.

Product details:
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-Purified from TCS., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.
Biological function summary
Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.
Pathways
Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.
Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.