Abcam, ab110277, Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7]

Size: 100µg
Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab110277) is a mouse monoclonal antibody detecting ATPase Inhibitory Factor 1/IF1 in Western Blot, Flow Cytometry, ICC/IF . Suitable for Cow, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5E2D7,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human,
Applications:WB, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab110277) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.
What is the molecular weight of ATPase Inhibitory Factor 1/IF1?
Anti-ATPase Inhibitory Factor 1/IF1 [5E2D7] (ab110277) specifically detects a band for ATPase Inhibitory Factor 1/IF1 (UniProt: Q03344) at a molecular weight of 12kDa.
Trusted by the scientific community
Anti-ATPase Inhibitory Factor 1/IF1 [5E2D7] (ab110277) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab110277) has been confirmed by Western blot testing in ATP5IF1 Knockout HAP1 cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification notes-The purity of ab110277 is near homogeneity, as judged by SDS-PAGE. ab110277 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATPase Inhibitory Factor 1 (IF1) also referred to as IF1 protein ATPIF1 or ATPase inhibitory factor 1 is a small protein with a mass of approximately 12 kDa. It is expressed primarily in mitochondria-rich tissues such as the heart liver and brain where it plays an important role in energy metabolism. IF1 is notable for its ability to bind to the F1 subunit of the mitochondrial ATP synthase inhibiting its ATPase activity under specific conditions.
Biological function summary
IF1 influences mitochondrial function by regulating ATP synthase in response to changes in the cellular environment. Under conditions of low cellular energy IF1 stabilizes and prevents hydrolysis of ATP by binding to the F1 catalytic domain of the enzyme. This regulation helps to conserve ATP critical for maintaining cellular viability during stress. IF1 can also interact with other mitochondrial proteins to form complexes that influence energy homeostasis in the cell.
Pathways
IF1 integrates into pathways involved in cellular energy management including oxidative phosphorylation and ATP synthesis pathways. It interacts significantly with proteins such as the mitochondrial F1Fo-ATP synthase complex. By modulating ATP synthase activity IF1 ensures efficient ATP production and maintains mitochondrial membrane potential important for proper cell function.
IF1 has associations with metabolic conditions and cancer. Abnormal expression or activity of IF1 correlates with mitochondrial dysfunction often observed in cancer cells where it affects cell proliferation. Additionally IF1 has links to cardiac conditions given its role in regulating heart metabolism. It relates to proteins like the cytochrome c oxidase influencing cellular respiration and affecting disease progression.