Abcam, ab11083, Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D]

Size: 50µL
Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) is a mouse monoclonal antibody detecting Slow Skeletal Myosin Heavy chain in Western Blot, IHC-P . Suitable for Rabbit, Rat . - Over 110 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:NOQ7.5.4D,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat, Rabbit,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human MYH7.P12883

Product details:
What is this antibody validated in?
Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Rabbit, Rat samples.
What is the molecular weight of Slow Skeletal Myosin Heavy chain?
Anti-Slow Skeletal Myosin Heavy chain [NOQ7.5.4D] (ab11083) specifically detects a band for Slow Skeletal Myosin Heavy chain (UniProt: P12883) at a molecular weight of 200kDa.
Trusted by the scientific community
Anti-Slow Skeletal Myosin Heavy chain [NOQ7.5.4D] (ab11083) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals.
Reviewed by scientists
Anti-Slow Skeletal Myosin Heavy chain [NOQ7.5.4D] (ab11083) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-Purified from Tissue culture supernatant., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The slow skeletal myosin heavy chain also known as anti-slow or anti-sluggish is an essential protein component of muscle tissue. This protein is identified as MYH7 chain or type D chain with a molecular mass around 220 kDa. It is predominantly expressed in slow-twitch muscle fibers which are found in skeletal muscles responsible for enduring low-intensity (endurance) activities. Myosin staining techniques often highlight the distribution and density of these fibers in muscle tissues providing insights into fiber-type composition.
Biological function summary
This myosin heavy chain participates in muscle contraction by interacting with actin filaments forming cross-bridges through ATP-dependent mechanisms. As a part of the sarcomeric structure MYH7 works within the thick filament complex contributing to the contractile force of slow-twitch muscles. This particular myosin isoform is important for maintaining muscle stamina and efficiency over prolonged activities providing the necessary mechanical support for the generation of force at a lower contraction speed compared to fast-twitch fibers.
Pathways
The slow skeletal myosin heavy chain is integral to the calcium signaling pathway that regulates muscle contraction. This protein interacts with troponin and tropomyosin to modulate the actin-myosin interaction in a calcium-dependent manner. Additionally it plays a role in the heart muscle contraction pathway linking it to proteins such as actin which is important for cardiac function and rhythmic contraction.
Slow skeletal myosin heavy chain mutations are linked to cardiomyopathy particularly hypertrophic cardiomyopathy and dilated cardiomyopathy. These conditions compromise heart muscle function leading to symptoms such as fatigue and shortness of breath. The MYH7-associated disorder is often observed alongside alterations in other sarcomeric proteins like troponin T which further disrupts muscle contraction and cardiac output. Understanding these connections can aid in developing interventions and treatments for muscle-related diseases.