Abcam, ab111648, Recombinant Human KAT2A / GCN5 protein

Size: 100µg
Recombinant Human KAT2A / GCN5 protein is a Human Fragment protein, in the 411 to 837 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q92830,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KAT2A also known as GCN5 is a lysine acetyltransferase enzyme with a molecular mass of approximately 92 kDa. It mainly acetylates histone proteins and affects transcriptional regulation by modifying chromatin structure. KAT2A/GCN5 is expressed in various tissues with significant expression in the nucleus of eukaryotic cells. Its role as a histone acetyltransferase places it as an important player in transcriptional activation by modifying the chromatin to a more open conformation facilitating access by transcription machinery.
Biological function summary
KAT2A/GCN5 participates in several cellular processes as part of larger protein complexes. It is a component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) and TFTC (TBP-free TAF-containing complex) both involved in transcription regulation. Through these complexes KAT2A influences gene expression by acetylating histone residues which in turn affects the recruitment of other transcriptional activators or repressors. This activity also links it to non-histone substrates that support DNA damage repair and cellular senescence.
Pathways
KAT2A/GCN5 is integrally connected to the regulation of transcription and chromatin remodeling pathways. It interacts closely with the transcription factor c-Myc assisting in cell cycle regulation and proliferation. Additionally its presence in the p53 signaling pathway highlights its involvement in controlling cell growth and apoptosis. KAT2A/GCN5 activity influences downstream events in these pathways by affecting the acetylation state of specific histones and non-histone proteins.
The dysregulation of KAT2A/GCN5 is linked to cancer particularly in breast and colorectal cancers. Its role in acetylating key proteins like c-Myc and affecting pathways like p53 can lead to unchecked cell growth when improperly regulated. Furthermore KAT2A is connected to neurodegenerative diseases through its interactions with other histone modifiers such as HDAC1 and HDAC2 implicating it in conditions where neural cell function or maintenance goes awry.