Abcam, ab111681, Anti-PITRM1 antibody

Size: 50µL
Rabbit Polyclonal PITRM1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PITRM1 aa 1-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PITRM1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.Q5JRX3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab111681 is purified by antigen-affinity chromatography., Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pitrilysin metallopeptidase 1 (PITRM1) also known as Presequence Protease or Mitochondrial Intermediate Peptidase (MIP) is a metalloprotease with an approximate molecular mass of 113 kDa. PITRM1 localizes mainly to the mitochondrial matrix where it plays an essential role in protein maturation and degradation of short peptide substrates especially mitochondrial targeting peptides that remain after precursor protein processing. The enzyme's primary function involves maintaining mitochondrial protein homeostasis which is critical for normal mitochondrial operation.
Biological function summary
PITRM1 functions as a non-processive metallopeptidase that cleaves peptides within the mitochondria after they have been imported and processed by mitochondrial processing peptidase (MPP). It does not form part of a larger complex but acts independently to ensure efficient degradation of cleaved presequence peptides. This activity helps prevent the accumulation of peptide fragments that could disrupt mitochondrial function safeguarding cellular energy production and metabolism.
Pathways
PITRM1 operates within the mitochondrial import pathway specifically contributing to the processing of mitochondrial precursor proteins. This enzyme acts in conjunction with proteins like MPP and allows the preparation of mitochondrial proteins for their functional roles. Additionally PITRM1 intersects the apoptosis pathway indirectly by influencing mitochondrial integrity and the proteolysis it modulates can affect proteins linked to cell death.
PITRM1 has been implicated in neurodegenerative diseases including Alzheimer's disease and a form of hereditary spastic paraplegia. Deficiencies or mutations in PITRM1 can lead to the buildup of amyloid-beta and other peptides as it normally degrades these toxic substrates. The enzyme's dysfunction affects mitochondrial function and is linked to proteins such as amyloid precursor protein (APP) and presenilins known contributors to Alzheimer’s pathology. Understanding PITRM1 function and its impact on peptide degradation offers insights into therapeutic targets for these conditions.