Abcam, ab111733, Anti-ATP6V1E1 antibody

Size: 50µL
Rabbit Polyclonal ATP6V1E1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1E1 aa 1-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP6V1E1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.P36543

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V1E1 also known as V-type proton ATPase subunit E1 is a component of the vacuolar ATPase (V-ATPase) complex which is key in cellular processes like acidification of intracellular organelles. This protein has a mass of approximately 31 kDa and is found extensively in the cytosol. ATP6V1E1 is expressed in various tissues including those in the kidney liver and brain indicating its widespread significance in cellular function.
Biological function summary
ATP6V1E1 plays a role in energizing the V-ATPase complex which contributes to proton translocation across intracellular membranes. The protein acts within the V1 domain of the V-ATPase a multi-subunit enzyme responsible for acidifying compartments such as endosomes lysosomes and the Golgi apparatus. This acidification is important for protein degradation receptor-mediated endocytosis and neurotransmitter uptake highlighting ATP6V1E1's role in maintaining cellular homeostasis.
Pathways
ATP6V1E1 is integrated into processes like the autophagy and endocytic pathways. It interacts with other proteins within the V-ATPase complex to regulate organelle pH and support vital functions such as lysosomal degradation. ATP6V1E1's interactions with proteins like ATP6V1A highlights its involvement in these metabolic pathways demonstrating how these components work together to regulate cellular energy and waste management.
ATP6V1E1 has connections to disorders including renal tubular acidosis and osteopetrosis. Mutations in this protein can disrupt the normal acidification process leading to these conditions. Through its role in the V-ATPase complex ATP6V1E1 is functionally linked to other subunits like ATP6V1A. Together they influence the pathophysiology of these diseases by impacting the regulatory mechanisms necessary for stable cellular pH and related metabolic activities.