Abcam, ab112995, Anti-Calnexin antibody [6F12BE10]

Size: 100µg
Anti-Calnexin antibody [6F12BE10] (ab112995) is a mouse monoclonal antibody detecting Calnexin in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Over 10 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:6F12BE10,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, ICC/IF, IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

Product details:
What is this antibody validated in?
Anti-Calnexin antibody [6F12BE10] (ab112995) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Calnexin?
Anti-Calnexin [6F12BE10] (ab112995) specifically detects a band for Calnexin (UniProt: P27824) at a molecular weight of 68kDa.
Trusted by the scientific community
Anti-Calnexin [6F12BE10] (ab112995) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-Calnexin antibody [6F12BE10] (ab112995) has been confirmed by Western blot testing in CANX Knockout HAP1 cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-ab112995 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%)., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: 99.98% HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.
Biological function summary
Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.
Pathways
Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.
Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.