Abcam, ab113150, Recombinant human PPP1A/PPP1CA protein

Size: 10µg
Recombinant human PPP1A/PPP1CA protein is a Human Full Length protein, in the 1 to 330 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass Spec, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:>3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 °C, pH 7.5, using 10 mM of substrate.,
Accession:P62136,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8.5Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% Manganese chloride, 0.002% PMSF

Product details:
This product was previously labelled as PPP1A

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein phosphatase 1 alpha also known as PPP1A or PPP1CA is a catalytic subunit of protein phosphatase 1 with a molecular weight of approximately 37 kDa. PPP1A plays a role in the dephosphorylation of serine/threonine residues in various proteins. It is expressed widely in human tissues with significant presence in the brain heart and skeletal muscle. PPP1A is essential for many cellular processes due to its widespread distribution and ability to interact with different substrate proteins.
Biological function summary
Protein phosphatase 1 alpha controls key aspects of cellular function. It often exists as part of complexes with other regulatory proteins which modulate its specificity and activity. These complexes participate in cell cycle regulation muscle contraction protein synthesis and glycogen metabolism. PPP1A's role in forming reversible phosphorylation networks highlights its importance in maintaining cellular homeostasis and responding to various signals.
Pathways
The role of PPP1A is vital in pathways such as the cell cycle and glycogen metabolism. It interacts with proteins like cyclin-dependent kinases to manage the cell division process and collaborates with glycogen synthase to regulate glycogen synthesis and breakdown. The involvement of PPP1A in these pathways showcases its versatility in influencing cellular metabolism and division processes.
Protein phosphatase 1 alpha is linked to cardiac diseases and cancer. Its dysregulation can influence heart muscle contractility contributing to cardiac hypertrophy and heart failure. Additionally abnormal PPP1A activity can impact tumorigenesis through interactions with cancer-related proteins like Akt. Understanding PPP1A's role in these diseases provides insights into potential therapeutic targets for treatment.