Abcam, ab115730, Anti-Glucose Transporter GLUT1 antibody [EPR3915]

Size: 20µL / 100µL / 1mL
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) is a rabbit monoclonal antibody detecting Glucose Transporter GLUT1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 320 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3915,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We recommend not to boil the samples after lysis to get desired WB bands.

Product details:
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) was first used in a scientific publication in 2012 and has been cited over 320 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) has been confirmed by Western Blot testing in Glucose Transporter GLUT1 knockout HepG2 cells (
ab280797
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) has 18 independent reviews from customers.
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) specifically detects Glucose Transporter GLUT1 (UniProt ID: P11166; Molecular weight: 55kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR3915 -
ab196357
Antibody clone EPR3915 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, HRP, Alexa Fluor
488, Alexa Fluor
594, PE, Alexa Fluor
405, APC, FITC (
ab195020
ab195021
ab195359
ab206360
ab209449
ab210438
ab316298
ab322306
GLUT1 significantly impacts inflammation by facilitating glucose uptake in immune cells, which is essential for their activation and function. GLUT1 activity can lead to chronic inflammation and tissue damage. GLUT1 is a key biomarker in IBD and immunometabolism. GLUT1 is a signature in Met-flow analysis. Antibody is top cited with over 428 publications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
Biological function summary
This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.
Pathways
GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.
GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.