Abcam, ab119442, Recombinant Human RACK1 protein

Size: 50µg
Recombinant Human RACK1 protein is a Human Full Length protein, in the 1 to 317 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P63244,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RACK1 or Receptor for Activated C Kinase 1 is an approximately 36 kDa protein that acts as scaffolding facilitating protein-protein interactions. It has a conserved WD40 repeat domain structure essential for its functions. RACK1 is ubiquitously expressed in various tissues including brain liver and lungs. It aids in assembling signaling complexes by anchoring signaling proteins contributing to various cellular responses.
Biological function summary
The function of RACK1 influences numerous cellular processes such as cell growth migration and apoptosis. It forms part of the ribosome serving a function in translation regulation. RACK1 participates in G-protein mediated signaling as well and binds to activated PKC (protein kinase C) isoforms promoting effective signaling pathways that affect cell behavior.
Pathways
Studies identify RACK1 within the MAPK and JAK/STAT pathways. In the MAPK pathway RACK1 works alongside proteins like ERK1/2 to mediate cellular responses to external stimuli. Within the JAK/STAT pathway its interaction with kinases influences the transcriptional activation of genes involved in immune responses and cell growth signaling emphasizing its role in regulating important cellular activities.
Researchers have linked RACK1 to certain cancers and neurodegenerative diseases. In cancer RACK1's interaction with PKC and other signaling proteins like Src can affect cell proliferation and angiogenesis features significant in tumor progression. Regarding neurodegenerative diseases RACK1's involvement in amyloid precursor protein processing connects it to Alzheimer's disease progression with potential interactions with proteins like presenilin highlighting its role in pathophysiology.