Abcam, ab119520, Human Superoxide Dismutase 1 ELISA Kit

Size: 1 x 96Tests
Human Superoxide Dismutase 1 ELISA Kit is a sandwich ELISA designed to quantify Human Superoxide Dismutase 1 with a sensitivity of 0.04 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.08 - 5 ng/mL - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.04 ng/mL,
Range:0.08 - 5 ng/mL,
Assay Platform:Microplate

Product details:
Human Superoxide Dismutase 1 ELISA Kit ab119520 is a sandwich ELISA to measure Human Superoxide Dismutase 1 in serum, plasma, cell culture supernatant, urine with a sensitivity of 0.04 ng/ml.
How the assay works
Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Superoxide Dismutase 1 ELISA Kit ab119520 protocol summary
1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary
The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.
Pathways
SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.
Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.