Abcam, ab119758, Anti-NNMT antibody [OTI3D8]

Size: 100µL
Anti-NNMT antibody [OTI3D8] (ab119758) is a mouse monoclonal antibody detecting NNMT in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, . - KO validated for confirmed specificity - Over 10 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI3D8,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, Flow Cyt (Intra), ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human Nicotinamide N-methyltransferase.P40261

Product details:
What is this antibody validated in?
Anti-NNMT antibody [OTI3D8] (ab119758) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, samples.
What is the molecular weight of NNMT?
Anti-NNMT [OTI3D8] (ab119758) specifically detects a band for NNMT (UniProt: P40261) at a molecular weight of 30kDa.
Trusted by the scientific community
Anti-NNMT [OTI3D8] (ab119758) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-NNMT antibody [OTI3D8] (ab119758) has been confirmed by Western blot testing in NNMT Knockout HeLa cell line,
ab265700

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-ab119758 is purified from TCS by affinity chromatography., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NNMT protein also known as Nicotinamide N-methyltransferase acts by catalyzing the methylation of nicotinamide using S-adenosylmethionine as a methyl donor. This enzyme has a mass of around 29 kDa and is expressed in various tissues including liver adipose tissue and certain cancer cells. The NNMT gene provides instructions for creating the protein that plays significant roles in metabolic processes and cellular homeostasis.
Biological function summary
NNMT impacts cell energy balance and gene expression regulation by influencing the levels of nicotinamide and methionine metabolites. This enzyme does not typically form part of a complex acting more so as an individual regulator of methylation reactions affecting cellular metabolism. Researchers find interest in NNMT for its role in modulating cellular growth and proliferation forming a link to both normal cellular functions and pathological conditions like cancer.
Pathways
NNMT has a significance in NAD+ metabolism and methionine salvage pathways. These pathways are essential in maintaining redox homeostasis and proper methylation status in cells. NNMT through its enzymatic activity interacts with SAM (S-adenosyl methionine) closely relating its function to methylation processes that involve other proteins such as SIRTs (Sirtuins) and NAMPT (Nicotinamide phosphoribosyltransferase) within these metabolic pathways.
NNMT shows relevance in cancer and obesity-related complications. The protein's upregulation in cancer types like breast and colorectal cancers links it to tumor development and progression. In obesity NNMT's involvement in adipose tissue might contribute to metabolic dysregulation. The enzyme also interconnects with proteins like insulin receptors and adiponectin highlighting its potential role in metabolic diseases and making it an interesting target for therapeutic interventions.