Abcam, ab119796, Anti-PFKP antibody [OTI1D6]

Size: 100µL
Mouse Monoclonal PFKP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate, Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PFKP.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI1D6,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PFKP.Q01813

Product details:
The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.
Dilute in PBS (pH 7.3) before use.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS, Storage buffer-pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PFKP also known as 6-phosphofructokinase platelet type functions as an important glycolytic enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate using ATP. This process shows the rate-limiting step in glycolysis. The PFKP protein weighs approximately 85 kDa. Expression occurs in various tissues with high presence in platelets and muscle tissues indicating its significant role in energy metabolism.
Biological function summary
PFKP plays an essential role in maintaining energy supply ensuring efficient glycolysis during high energy demands. It associates with other isoforms PFKM (muscle) and PFKL (liver) to form a tetrameric complex which can adapt its activity based on the energy needs of the cell. This adaptability defines its contribution to cellular metabolism regulation considering energy substrates and signaling molecules.
Pathways
The function of PFKP ties closely to glycolysis and the larger metabolic pathways managing energy production such as the tricarboxylic acid (TCA) cycle. Within these pathways PFKP interacts with other metabolic regulators including AMP-activated protein kinase (AMPK) which influences cell metabolism in response to energy availability. Connections with PFKL and PFKM reveal that PFKP's activity relates to the overall glycolytic flux and energy balance.
PFKP's alterations contribute to metabolic conditions like cancer and diabetes. Its dysregulation in cancer cells supports the Warburg effect where tumor cells prefer glycolysis. The link with metabolic proteins such as AMPK also indicates a potential role in diabetes as both disorders involve shifts in energy metabolism. Understanding PFKP's impact on these diseases helps in developing therapeutic strategies targeting metabolic pathways.