Abcam, ab119952, Anti-pan Actin antibody [4A4]

Size: 100µL
Mouse Monoclonal alpha smooth muscle Actin antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human, Rat, Mouse, African green monkey, Recombinant fragment - Human samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4A4,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:WB, IHC-P, ELISA, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACTA2 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P62736,
Specificity:Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein but this product will also detect Gamma actin, and likely other actin family members too.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The actin protein sometimes referred to as "pan actin" is an important component of the cytoskeleton and is fundamental in maintaining cell structure and integrity. Alternately called beta-actin or gamma-actin depending which variant is expressed this protein has a molecular weight of approximately 42 kDa. Actin is ubiquitously expressed in almost all eukaryotic cells where it forms dynamic networks that support the plasma membrane assist in cell movement and mediate intracellular transport.
Biological function summary
Actin polymerizes to form filaments (F-actin) from its monomeric forms (G-actin) within the cell. These filaments are critical for constructing the cytoskeleton and anchor cellular structures in place. Actin functions within the actin-myosin complex which facilitates muscle contraction and movement in non-muscle cells. Through this complex actin provides essential support in cellular processes such as cytokinesis and vesicle movement.
Pathways
Actin is important for pathways that control cell motility and structural rearrangements. It plays a significant role in the Rho-GTPase signaling pathway which regulates actin cytoskeleton dynamics. Actin interacts with other important proteins such as myosin and tropomyosin to facilitate these activities. Another important pathway involving actin is the Wnt signaling pathway where it aids in the regulation of cell proliferation and differentiation.
Actin is linked to conditions such as cancer metastasis and muscular dystrophies. In cancer actin's role in cell motility and invasion contributes to the metastatic spread of tumors. Actin dysregulation is also implicated in certain muscle disorders where mutations affect the formation and function of muscle fibers. In these contexts actin closely connects with dystrophin an important protein in muscle tissue integrity and any disruption can lead to disease manifestation.