Abcam, ab120463, Dynole® 34-2, dynamin I and dynamin II inhibitor

Size: 5mg
MW 408.6 Da, Purity >99%. Potent, cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.3 and 14.2 μM for inhibition of dynamin I and II GTPase, respectively). Targets the GTPase domain at an allosteric site. Potently inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 5 and 41.1 μM, respectively). 15-fold more active than Dynasore (ab120192) against dynamin I.
Key facts
CAS number:1128165-88-7,
Purity:>99%,
Form:SolidSee storage information,
Molecular weight:408.6 Da,
Molecular formula:C25H36N4O,
PubChem:44157463,
Nature:Synthetic,
Solubility:Soluble in DMSO to 100 mM,
Biochemical name:Dynole 34-2,
Biological description:Potent, cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.3 and 14.2 μM for inhibition of dynamin I and II GTPase, respectively). Targets the GTPase domain at an allosteric site. Potently inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 5 and 41.1 μM, respectively). 15-fold more active than Dynasore (ab120192) against dynamin I.,
Canonical smiles:CCCCCCCCNC(=O)C(=CC1=CN(C2=CC=CC=C21)CCCN(C)C)C#N,
Isomeric smiles:CCCCCCCCNC(=O)/C(=C/C1=CN(C2=CC=CC=C21)CCCN(C)C)/C#N,
InChi:InChI=1S/C25H36N4O/c1-4-5-6-7-8-11-15-27-25(30)21(19-26)18-22-20-29(17-12-16-28(2)3)24-14-10-9-13-23(22)24/h9-10,13-14,18,20H,4-8,11-12,15-17H2,1-3H3,(H,27,30)/b21-18+,
InChiKey:MYIMRONQBLZJFI-DYTRJAOYSA-N,
IUPAC Name:(E)-2-cyano-3-[1-[3-(dimethylamino)propyl]indol-3-yl]-N-octylprop-2-enamide

Product details:
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store under desiccating conditions, The product can be stored for up to 12 months

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dynamin 1 and Dynamin 2 are important proteins playing an important mechanical role in cellular processes. Dynamin proteins are large with masses of about 96 kDa for Dynamin 1 and 100 kDa for Dynamin 2. They function as GTPases driving membrane constriction and fission. These processes help in membrane trafficking such as endocytosis. Both types of Dynamin are present in various tissues with Dynamin 1 being expressed mainly in the brain and neurons while Dynamin 2 is expressed in non-neuronal cells.
Biological function summary
Dynamins are involved in essential tasks within cells specifically mediating membrane scission. Dynamin 1 along with Dynamin 2 forms helical structures around the neck of budding vesicles contributing to their detachment from the plasma membrane. These proteins work closely with actin and microtubule networks ensuring proper cytoskeleton-dependent trafficking. Dynamins can also interact with a complex of proteins including amphiphysin and endophilin enhancing their function.
Pathways
Dynamins play significant roles in synaptic vesicle recycling and receptor-mediated endocytosis. These pathways are important for neurotransmitter release and nutrient uptake. In these contexts Dynamins interact with proteins like clathrin and sorting nexin. The GTPase activity of Dynamins is fundamental to their action in these pathways facilitating vesicle budding and fusion events.
Disruptions in Dynamin function are linked to cancer and neurological diseases. Mutations in Dynamin 2 can cause Charcot-Marie-Tooth disease a hereditary neuropathy affecting peripheral nerves. Additionally altered expression or function of Dynamins relates to cancer types like glioblastoma often through interactions with proteins such as EGFR which can exacerbate tumor progression and chemoresistance.